Correlation of MSR1 single nucleotide polymorphism with susceptibility, clinical characteristics and prognosis of lung cancer

Abstract

Objective To investigate the relationship between single nucleotide polymorphism (SNP) of macrophage scavenger receptor 1 (MSR1) and susceptibility, clinical features, pathological features, chemotherapy outcomes, prognosis of lung cancer. Methods 99 patients with lung cancer hospitalized in Ruijin Hospital of Shanghai Jiaotong University from January 2015 to June 2016 were recruited, and 100 healthy people were collected as control.DNA was amplified by polymerase chain reaction, then genotype polymorphism of MSR1(rs13306550) was detected. Results There were significant differences in the genotype distribution of MSR1 (rs13306550) between the two groups (χ2=10.077, P=0.002), the patients with AG genotype had obviously higher risk to lung cancer than that with AA genotype (P=0.013, OR=13.66, 95%CI: 1.74-107.16). There was no significant difference in genotype distribution among different pathological types in lung cancer group (χ2=0.801, P=0.805). There were significant differences in genotype distribution among different T stages (χ2=9.451, P=0.021), the AG genotype in T4 stage was significantly more susceptible than AA genotype(χ2=8.809, P=0.008). There was no significant difference in the genotype distribution of patients with lymph node metastasis /distant metastasis or not.In lung cancer group, 86 patients with non-small cell lung cancer (NSCLC) were treated with platinum-based chemotherapy, and their clinical responses were compared.There was no significant difference in genotype distribution between complete response+ partial response+ stable disease group and progressive disease group.Multivariate logistic regression analysis showed that there was no correlation of chemotherapy outcomes with genotype, age, gender, smoking status, histopathological type, lymphatic metastasis, and distant metastasis.The chemotherapy outcomes of patients in T4 stage were better than those in other stages (P=0.018, OR=0.24, 95%CI: 0.08-0.79). Using progression free survival as the observed variable, there was no significant difference in the survival prognosis between AA and AG genotype. Conclusions The MSR1 (rs13306550) gene polymorphism is associated with lung cancer susceptibility, AG genotype is associated with the risk of lung cancer, but SNP is not associated with the histological type, lymphatic metastasis, distant metastasis, and prognosis of lung cancer, and the chemotherapy outcomes of NSCLC patients.The MSR1 (rs13306550) gene SNP is associated with T stage of lung cancer, AG genotype is significantly more susceptible in T4 stage than AA genotype.The platinum-based chemotherapy outcomes of NSCLC patients in T4 stage are better than those in other stages. Key words: Macrophage scavenger receptor 1 gene; Single nucleotide polymorphism; Lung cancer; Susceptibility; Prognosis