Association of Leptin gene-2 548 G/A polymorphism with the susceptibility and prognosis of non-small cell lung cancer

Abstract

Objective To explore the association of Leptin gene-2 548 G/A polymorphism with the susceptibility and prognosis of non- small cell lung cancer(NSCLC). Methods A total of 162 patients with NSCLC and 200 controls were enrolled in the case-control study. The genotypes were detected using reaction-restriction fragment length polymorphism(PCR-RFLP). Relevant clinicopathological data of selected cases were collected and analyzed. Results The frequencies of AA, GA and A allele in cases(AA:35.2%;GA:46.3%;A:58.3%)were higher than that in controls(AA:16.5%;GA:40.0%;A:41.7%),and significant differences were revealed[odds ratio (OR)= 5.009,95% confidence interval(CI):2.758- 9.097,P< 0.01;OR= 2.719, 95% CI:1.615 - 4.578, P< 0.01;OR= 2.436, 95% CI:1.804- 3.289, P< 0.01], respectively. The frequencies of AA+ GA genotype(58.3%) was found higher than GG genotype(33.3%)in NSCLC inⅢ+ IV stage, likewise, the frequencies of AA+ GA genotype(62.1%) were also found higher than GG genotype(40.0%) in NSCLC with lymph node metastasis, significant differences were also showed (both P< 0.05), and the survival of individuals carrying GA+ AA(37.7±1.1) genotype was markedly reduced compared to that carrying GG(45.9±2.9)genotype(χ2= 8.215,P< 0.01). Conclusion The present study suggests that the Leptin gene-2 548 G/A polymorphism may be associated with the susceptibility to NSCLC, and appear to jointly contribute to predicting a poor prognosis. Key words: Non-small cell lung cancer; Leptin; Polymorphism