Genetic variability among Fusarium oxysporum isolates from melon (Cucumis melo) in Qazvin province, Iran

Mohammadreza Mahdikhani

doi:10.19071/jhcbt.2016.v2.2996

Abstract

Fusarium wilt, caused by Fusarium oxysporum f. sp. melonis (Fom), the soil-borne fungus that can due to more losses in yield of melon plants. Fusarium is a variable fungi in terms of morphology, symptom production, pathogenicity and wide host range. From 2013 to 2015, 120 isolates of F. oxysporum, F. acuminatum, F. graminearum, F. proliferatum and F. solani from melon, were collected from disease suspected plants from the field and defined for pathogenicity in our melon plants. Then, a sort of those were elected for phylogenetic analysis. In the beginning, the Fusarium isolates were classified based on morphology and identifications were authenticated based on sequence data from, EF1α and DNA, and then were used to determine whether Fusarium variability is relevant to geographic origin and pathogenicity. Neighbor-joining analyses datasets indicated some clades based on geographic origin but there is not even a single clades including solely of pathogens. Because of the factors affecting in pathogenicity are variable, we should be considered them in future studies. Due to the presence of FOM and some nonpathogenic isolates in almost all clades, it is obvious that Fusarium oxysporum f. sp. melonis is not monophyletic.

Highlights

Fusarium wilt is a soilborne disease which is the result of Fusarium oxysporum Schl
Host resistance is the best way for disease management.To host plant resistance breeding, we must know if exists the variability within the pathogen population.the present research study was performed to explain variation among F. oxysporum isolated from melon using DNA sequencing of translation elongation factor 1α (TEF1α)
Based on the morphological characteristics of isolates from farmlands, we identified F.oxysporum,Fusarium acuminatum, Table 1: Geographic origin and pathogenicity on melon seedling of Fusarium oxysporum, Fusarium acuminatum, Fusarium graminearum, Fusarium proliferatum and Fusarium solani cultured from melon, tomato and watermelon

Summary

Introduction

Fusarium wilt is a soilborne disease which is the result of Fusarium oxysporum Schl. This fungi is one of the most important pathogens, which is causes wilting in some crops such as cantaloupe, cucumber, wax gourd, muskmelon, watermelon, and other types of melon. Host resistance is the best way for disease management.To host plant resistance breeding, we must know if exists the variability within the pathogen population.the present research study was performed to explain variation among F. oxysporum isolated from melon using DNA sequencing of translation elongation factor 1α (TEF1α). The goal of this study was to determine phylogenetic relationship among F. oxysporum isolates, gathered from melon plants, and determine diversity correlates with location origin and pathogenicity

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Conclusion