Abstract
The genetic location of the cet gene, mutation of which renders Escherichia coli tolerant to colicin E2, has been determined by crosses involving conjugation and P1 transduction. The gene order obtained was: dnaD-dra-drm-serB-cet-thr, in constrast to other reports which have placed cet to the left of serB. In addition, the results of one cross indicated that cet probably lies to the right of trpR, i.e., serB-trpR-cet-thr. Co-transduction frequencies indicated that the sites of the cet mutations were located in similar positions in both CetB−(E2-tolerant, UV-resistant) and CetC−(E2-tolerant, UV-sensitive) strains. Cet mutations were found to be dominant to the wild-type allele in partial diploids.
Published Version
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