Genetic structure and diffusion of population of <i>Coilia ectenes taihuensis</i> in Lake Nansi inferred from the mitochondrial control region

Abstract

为推断南四湖湖鲚种群来源,采用线粒体控制区(D-loop)基因序列测定技术,对3个不同水域湖鲚(Coilia ectenes taihuensis)种群的遗传结构特征及其变异进行研究,并分析它们之间的系统发育关系. 结果表明3个不同湖鲚种群的平均单倍型多样性和核苷酸多样性分别为0.9646和0.0097,除3个单倍型共享外,不同地理单元中具有较高的单倍型多样性.AMOVA和成对F_st值分析均表明,不同湖泊群体间未发现明显的遗传分化,各地理单元中的单倍型相互散布在不同的种群中.系统发育分析结果显示,不同湖泊湖鲚种群的单倍型形成3个分支,但每一地理群体没有显示出明显的地理位置与单倍型间的对应关系.结合南四湖历史资料和当前渔业监测结果,推测湖鲚为2002年实施长江向南四湖应急补水时洪泽湖湖鲚迁移扩散进而在南四湖建群的.;The genetic structure and genetic variation of three populations of Coilia ectenes taihuensis in different lakes are investigated using the mitochondrial control region gene. Results show that the hapotype diversity(h) and mean nucleotide diversity(π) of the populations of different lakes are 0.9646 and 0.0097, respectively. 30 hapotypes were detected out of 45 individuals from the three populations. Only three of these haplotypes were shared among three lakes which contributed much to the genetic diversity. The AMOVA analysis and the pairwise F_st values revealed no significance among three populations of C. ectenes taihuensis. NJ and Bayesian analyses revealed that individuals of three populations formed three clades with poor support, but no clear relationships between haplotypes and geographical regions. We inferred that C. ectenes taihuensis populations in Lake Nansi originate from lakes in the Yangtze River by the ecological urgent water diversion in 2002.