Abstract
Ants are important factor in ecosystem. They are greatly affected with environmental changes and human interference. Ants biomass do not look a lot like species richness and so their genetic information is valuable. Myrmicaria brunnea, harvester ant and Leptogenys chinensis, long legged ant used in study as representatives of Myrmicinae and Ponerinae respectively. RAPD technique is most common, reliable and cost-effective for study of genetic variability among different species. DNA marker was used for assessment of genetic integrity and variation among Myrmicaria brunnae and Leptogenys chinensis. Three RAPD markers, as P1, P2 and P3 generated 151 bands. M. brunnae and L. chinensis generated 66 and 85 bands respectively. Maximum polymorphic bands were generated by P2 primer (37) while, maximum monomorphic (17) bands were generated by primer P1. Study of monomorphic and polymorphic bands were carried out and statistical data generated were used to construct phylogenetic tree and fluorograms. M. brunnae and L. chinensis were found linked together at about 0.4 genetic distance.
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