Comparison of differentiation capability of RAPD and SSR markers in commercial barley (Hordeum vulgare L.) cultivars

Abstract

The polymorphic RAPD and SSR markers were used for distinguishing of commercial barley (Hordeum vulgare L.) cultivars and for comparison of differentiation capability of RAPD and SSR techniques. Fifteen of the 36 arbitrary used 10-oligonucleotide RAPD primers produced polymorphic RAPD markers. Two from twenty-three cultivars were undistinguished by generated RAPD markers. Jaccard’s coefficients of genetic similarity based on RAPD analyses varied from 0.250 to 1.0. Five SSR-based oligonucleotide primers used produced polymorphic, scorable, and reproducible patterns. Ali 23 cultivars were distinguished each of others by SSR markers. Jaccard’s coefficients of genetic similarity based on the SSR analyses varied from 0.0 to 0.833. The differentiation capability of two PCR-based techniques compared on the set of 23 commercial barley cultivars revealed that differentiation effectivity of SSR technique was more than seven times higher in comparison with the RAPD technique. The SSR primers were used also for differentiation of a second set of 40 spring and winter barley cultivars. They differentiated into 35 groups by SSR analyses. Higher genetic similarity was detected in subgroup of the winter barley cultivars.