Abstract
The polymorphic RAPD and SSR markers were used for distinguishing of commercial barley (Hordeum vulgare L.) cultivars and for comparison of differentiation capability of RAPD and SSR techniques. Fifteen of the 36 arbitrary used 10-oligonucleotide RAPD primers produced polymorphic RAPD markers. Two from twenty-three cultivars were undistinguished by generated RAPD markers. Jaccard’s coefficients of genetic similarity based on RAPD analyses varied from 0.250 to 1.0. Five SSR-based oligonucleotide primers used produced polymorphic, scorable, and reproducible patterns. Ali 23 cultivars were distinguished each of others by SSR markers. Jaccard’s coefficients of genetic similarity based on the SSR analyses varied from 0.0 to 0.833. The differentiation capability of two PCR-based techniques compared on the set of 23 commercial barley cultivars revealed that differentiation effectivity of SSR technique was more than seven times higher in comparison with the RAPD technique. The SSR primers were used also for differentiation of a second set of 40 spring and winter barley cultivars. They differentiated into 35 groups by SSR analyses. Higher genetic similarity was detected in subgroup of the winter barley cultivars.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
