Genetic polymorphism of GHR|Ssp and Pit1|Stu1 loci of twin and multiple births in local peranakan Ongole cattle

Abstract

Twin or multiple births may happen in beef cattle. Growth family genes directly control on growth traits, but also can affect on fertility of animal. This study aimed to identify genetic polymorphisms of GHR and Pit1 genes in local Peranakan Ongole (PO) cattle from twin and multiple births (M) compared to single birth (S). PO cattle as samples originated from four provinces of South Kalimantan, South Sumatra, Central Java and East Java. SNP variants of the GHR|Ssp locus at exon 8 (A/T) and Pit1|Stu1 locus at exon 3 (C/T) were analyzed by PCR RFLP technique. Allelic frequenciest of the GHR exon 8 g.914T>A locus of the two birth types were high for T allele (M = 78.1–100%, S = 71.4–100%) over A allele. Meanwhile the Pit1 exon 3 c.577C>T locus was monomorphic for the finding only C allele (100 %) without T allele. Some genetic indexes were also calculated for heterozygosity observation (Ho), heterozygosity expectation (He), effective number of alleles (Ne), fixation index (Fis) and polymorphism of information content (PIC). These values were not different between single and multiple births. The conclusion was that allelic frequenciest and genetic index values were almost similar between the two birth types of the local PO cattle.