Abstract
Monotheca buxifolia is an ethnomedicinally and economically important threatened fruit bearing plant species in Malakand Division Pakistan. The genetic diversity among the 92 various genotypes of Monotheca buxifolia was carried out using sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE) method. A considerable amount of inter districts genetic diversity (66.70%) was observed among the genotypes of M. buxifolia. Protein profiling was conducted on 12% gel electrophoresis. A total of 6 protein bands were observed in M. buxifolia genotypes. SDS-PAGE practice is a convenient scheme for the examination of both genetic diversity and relationship. Particularly, L-4 and L-5 were monomorphic in the inter districts Monotheca buxifolia genotypes and was recognized as species specific. The remaining other loci were polymorphic. In this investigation, the high inter and intra- districts specific diversity was observed demonstrating SDS-PAGE is an authoritative procedure for categorizing the genetically diverse germplasms in M. buxifolia. The findings from this study could be useful in the identification and selection of suitable M. buxifolia genotypes for future conservation programmes. Today, there is still a need to examine the genetic diversity and protect genetic resources, in particular wild species, for possible benefits in plant conservation programmes. To the best of our knowledge, this is the first ever report that addresses genetic variability in M. buxifolia.
Highlights
In this study we examined the genetic diversity of Monotheca buxifolia, a species threatened in Malakand Division, Pakistan
The region II (R-II) is comprised of all 23 genotypes collected from District Buner whereas the region III (R-III) and region IV (R-IV) are comprised of the genotypes collected District Dir lower and District Chitral respectively
Intra and inter Districts genetic diversity within the genotypes M. buxifolia The overall inter district (Swat, Buner, Dir lower and Chitral) genetic diversity among 92 genotypes of M. buxifolia is shown in Table 2 and remarkably, Locus 4 and 5 (L-4 and L-5) were present in the total genotypes of M. buxifolia and were considered as monomorphic loci
Summary
Monotheca buxifolia is an important ethnomedicinal, fruit-bearing evergreen tree with broad leaves, mostly growing as wild in nature, this species belongs to the family Sapotaceae (Khan et al, 2010; Ullah et al, 2016) This species is mainly found in Pakistan (main center KP province and Northern Pakistan), found in the hilly areas of Afghanistan, Akhdhar range of Northern Oman and Southwest Saudi Arabia near Zahran al Janub (Shahina and Martin, 1998; Khan et al, 2010).
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.