Gene frequencies of MN blood group in Heyuan, Guangdong

Abstract

Objective To investigate the gene frequencies of MN blood group in Heyuan,Guangdong.Methods 1000 random blood donors from Heyuan were detected for MN blood group by serologic technique.The gene types of doubt samples were detected bv polymerase chain reaction-sequence specific primers (PCR-SSP) and the gene frequencies were statistically analyzed at the same time.Results Genotyping results and serotyping results were in agreement.The phenotype frequencies of MM,MN,and NN were 0.316,0.493,and 0.191,respectively.The gene frequencies of M and N were 0.5625 and 0.4375,respectively in Heyuan,Guangdong.Chi-Square test indicated the distribution of genotypes agreed with Hardy-Weinberg equilibrium laws.The mismatch probability of M and N antigen was 0.3711 in random clinical blood transfusion.Conclusions In this investigation,the databases of the MN blood group's gene frequencies were supplemented lacuna in Heyuan,Guangdong and also provided a basis for clinical blood transfusion. Key words: MN blood group; gene frequency; Gene typing; Heyuan, Guangdong