Study on Genotype of ABO Blood Group System in Fuzhou Han Population

Abstract

Objective To study the distribution characteristics and regularity of ABO blood group genotype of Han population in Fuzhou.Methods From 12 January 2011 to 23 May 2011,a total of 450 Han blood donors who donated blood in Fujian Provincial Blood Center were selected in the study randomly.ABO genotype of all the donors was analyzed by polymerase chain reaction-sequence specific primer (PCR-SSP).Results In 450 unrelated individuals of Fuzhou Han population,five alleles of A1 (21.78％),A205 (0.67％),B (19.33％),O1 (32.78％),O2 (25.44％) were detected,but no O3 allele was discovered; twelve genotypes were found,consisting of A1O1 (14.00％),A1O2 (11.11％),A1A1 (4.22％),A1A205 (1.11％),BB (3.56％),BO1(12.00％),BO2 (10.44％),O1O1 (10.44％),O1O2 (18.67％),O2O2 (5.33％),A1B (8.89％),A205B (0.22％).The difference was not statistically significant between observed value and expected value in different genotypes (x2 =13.5339,P＞0.05).The genotype frequency distribution of this group was in good agreement with Hardy-Weinberg equilibrium.Conclusions Corresponding with ABO genotype,the distribution characteristics of ABO blood group in Fuzhou Han population was O＞A＞B＞AB,the ABO gene frequency distributions from high to low were O1,O2,A1,B,A205.A2 allele had its own characteristic,dominated by A205. Key words: Han population; ABO blood group; PCR-SSP; Genotype