Gelatin/Chitosan Bilayer Patches Loaded with Cortex Phellodendron amurense/Centella asiatica Extracts for Anti-Acne Application.

Chi-Wen Kuo,Chiung-Hua Huang,Ming-Hsien Li,Yi-Fang Chiu,Min-Hua Wu,Wan-Sin Chen,Cheng-Nan Wu

doi:10.3390/polym13040579

Chi-Wen Kuo, Chiung-Hua Huang + Show 5 more

Open Access

https://doi.org/10.3390/polym13040579

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Abstract

Acne is a chronic inflammatory skin disease that often occurs with anaerobic Propionibacterium acnes (P. acnes). Anti-acne patches, made of hydrocolloid or hydrogel, have become a popular way of topical treatment. The outer water-impermeable layer of commercial patches might create hypoxic conditions and promote P. acnes growth. In this study, gelatin/chitosan (GC) bilayer patches were prepared at different temperatures that included room temperature (RT), −20 °C/RT, and −80 °C/RT. The most promising GC bilayer patch (−80 °C /RT) contained a dense upper layer for protection from bacteria and infection and a porous lower layer for absorbing pus and fluids from pimples. The anti-acne bilayer patch was loaded with Cortex Phellodendri amurensis (PA) and Centella asiatica (CA) extracts. PA extract could inhibit the growth of P. acnes and CA extract was reported to improve wound healing and reduce scar formation. Moreover, the water retention rate, weight loss rate, antibacterial activity, and in vitro cytotoxicity of the patches were investigated. The porous structure of the patches promoted water retention and contributed to absorbing the exudate when used on open acne wounds. The GC bilayer patches loaded with PA/CA extracts were demonstrated to inhibit the growth of P. acnes, and accelerate the skin fibroblast cell viability. Based on their activities and characteristics, the GC bilayer patches with PA/CA extract prepared at −80 °C/RT obtain the potential for the application of acne spot treatment.

Highlights

The CCD-SH68 fibroblasts were incubated with various concentrations of Centella asiatica (CA) and Phellodendri amurensis (PA) for 48 h (Figure 1)
Based on the result of the cytotoxicity assay, concentrations of CA extracts in the range of 75–600 μg/mL were used for further experiments
The GC bilayer patch that was constructed at −80 ◦ C/room temperature with the addition of 1 mg/mL of PA and 2.4 mg/mL of CA demonstrated effective antibacterial activity and accelerated skin fibroblast cell viability

Summary

Introduction

Acne is a chronic inflammatory skin disease associated with comedones, papules, pustules, and nodules. It is a common problem affecting many teenagers. Acne pathogenesis is multifactorial and includes increased sebum and inflammation [1]. Propionibacterium acnes colonizes pilosebaceous follicles and is a major factor in the inflammatory reaction that occurs with acne vulgaris. P. acnes is a Gram-positive human skin commensal microorganism that prefers anaerobic growth conditions and is involved in acne pathogenesis [2]

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