Abstract
Human saliva from secretors of groups A, B, O and AB was gel filtered on Sephadex G-200 and G-100 columns, and on thin layers and the eluates were tested for A, B and H antigens by the haemagglutination inhibition method. A main excluded fraction (fraction 1) was detected in all the samples examined. A part of this fraction was also excluded from Sepharose 4B. The molecular weight of this fractions can be assumed to be between 2·10 5 and 5·10 6. In some samples of saliva, one or two group-specific subfractions were identified (fractions 2 and 3). Fraction 2 is excluded from Sephadex G-50 and probably has a molecular weight between 10 000 and 13 000. Fraction 3 is dialysable and has a lower molecular weight, probably no greater than 1500–2000. Fraction 1 is water-soluble and can be precipitated in alcohol, while fractions 2 and 3 are both water- and alcohol-soluble.
Published Version
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