Abstract
Glycolipids were isolated from human adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The lipid-bound sialic acid was about 234 μg/g fresh tissue. The ganglioside fraction contained two major gangliosides which accounted for 93% of the total lipid-bound sialic acid. They were identified as GM 3, N-acetylneuraminylgalactosylglucosylceramide and GD 3, N-acetylneuraminyl N-acetylneuraminylgalactosylglucosylceramide on the basis of cochromatography with authentic standards, sugar composition analysis, and neuraminidase digestion. GM 3, N-acetylneuraminylgalactosylglucosylceramide and GD 3, N-acetylneuraminyl N-acetylneuraminylgalactosylglucosylceramide occurred in a ratio of approximately 3 : 2, and the ratio seemed to be rather constant irrelevant of age and sex differences. The neutral glycolipid fraction consisted of GL 1a, glucosylceramide (18%), GL 1b, galactosylceramide (23%), GL 2a, lactosylceramide (27%), GL 3, digalactosylglucosylceramide (20%), and GL 4, globoside (12%). The major fatty acids of all these glycolipids were 16 : 0, 18 : 0, 22 : 0, 24 : 0 and 24 : 1.
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