Gambaran Histopatologi Selaput Lendir Kantung Empedu Sapi Bali yang Terinfeksi Cacing Fasciola gigantica (HISTOPATOLOGICAL ILLUSTRATION OF GALL BLADDER MUCOUS MEMBRANE INFASCIOLA GIGANTICA INFECTED BALI CATTLE)

I Made Kardena,Elyda Elyda,I Dewa Made Adhiwitana,Ida Bagus Oka Winaya,I Ketut Berata,Anak Agung Ayu Mirah Adi

doi:10.19087/jveteriner.2016.17.1.16

Abstract

This study aims were to describe lesions and their intensities of histopathological changes in gallbladder mucosa of bali cattle that infected with Fasciola gigantica. As many as 42 gall bladers of balicattle were used in this studi, i.e: 35 samples were infected and sefen were non-infected with F.gigantica.The samples were randomly gathered from Pesanggaran slaughter house in Denpasar. The gall bladderswere evaluated macroscopically and prepared for microscopically examination using haematoxylin andeosin staining.The result showed that there were histopathological changes observed in the mucosa of thegall bladder:necrosis cells of gall bladder with intencity 100%, hemorrhage 68.5%, hyperplasia serousgland and mucous hypertrophy gland 100%, collagen in filtration 100%, fibroblast proliferation 91.4%,and infiltration of inflammation cells, 74.2%. Histopathological changes in gallbladders mucous of balicattle infected F.gigantica experiencing severe lesons with frequency percentage above 50%.

Highlights

Fasciolosis adalah penyakit parasitik disebabkan oleh cacing dari genus Fasciola.Fasciolosis di Indonesia secara umum disebabkan oleh Fasciola gigantica
This study aims were to describe lesions and their intensities of histopathological changes in gall bladder mucosa of bali cattle that infected with Fasciola gigantica
As many as 42 gall bladers of bali cattle were used in this studi, i.e: 35 samples were infected and sefen were non-infected with F.gigantica

Summary

METODE PENELITIAN

Sebanyak 42 sampel kantung empedu sapi bali yaitu 7 sampel negatif (-) dan 35 sampel positif (+) terinfeksi cacing F.gigantica diambil secara acak dari Rumah Potong Hewan (RPH) Pesanggaran. Pengambilan seluruh sampel dilakukan selama satu minggu. Penentuan infeksi fasciolosis dilakukan dengan pengamatan makroskopis organ hati sapi bali yang terinfeksi cacing hati pada saluran dan kantung empedunya. Sampel organ tersebut selanjutnya difiksasi dengan larutan netral bufer formalin 10%. Kemudian dilakukan pembuatan preparat histologi dengan pewarnaan hematoksilin dan eosin (HE). Perubahan histopatologi selaput lendir kantung empedu diamati dengan mikroskop cahaya dengan perbesaran 100 dan 400 kali. Hasil pengamatan terhadap histopatologi selaput lendir kantung empedu dianalisis dan disajikan secara deskriptif kualitatif, sedangkan analisis intensitas perubahan histopatologi selaput lendir kantung empedu dilakukan secara deskriptif kuantitatif. Persentase lesi dihitung berdasarkan banyak jumlah sampel yang mengalami perubahan dibagi sampel keseluruhan dikali 100%

HASIL DAN PEMBAHASAN

Findings

DAFTAR PUSTAKA