Abstract
Studies were undertaken to determine the effect of method of radioiodination, antisera and sera on levels of endogenous gonadotropinreleasing hormone (GnRH)-like activity in serum as quantified by radioimmunoassay. GnRH was radioiodinated by the chloramine T or lactoperoxidase procedure and purified on columns of Sephadex G-25 (0.8 × 20 cm, eluted with 0.0lN acetic acid) or on columns of QAE Sephadex G-25-120, an anion exchange resin (0.6 × 25 cm, eluted with 0.1M borate buffer, pH 9.2, containing 0.1% gelatin). Unlabeled GnRH, monoiodo-GnRH and diiodo-GnRH were separated on the QAE Sephadex columns. Levels of immunoassayable GnRH-like activity in sera of ewes were lower (P < 0.05) when antiserum R-42 (high affinity) was used for quantification than when antiserum R-31 (low affinity) was used. Furthermore, levels of immunoassayable GnRH-like activity in sera were lower when monoiodo-GnRH from the lactoperoxidase radioiodination was used for quantification than when other radioiodinated preparations were u...
Published Version
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