Fungi Dekomposer Penghasil Enzim Ekstraseluler Lakase, Mangan Peroksidase, dan Lignin Peroksidase dari Kawasan Kebun Raya Bogor: Isolasi, Seleksi, Identifikasi dan Kajian Aktivitas Enzimnya

Abstract

This study aimed to obtain the candidate isolates of superior decomposer from several plant block areas in the Bogor Botanic Gardens (KRB). Those candidates were expected to be able to utilize in the fermentation process of solid organic waste. The superior decomposer fungi candidates were selected based on their abilities to produce extracellular enzymes (laccase - Lac, manganese peroxidase - MnP, and lignin peroxidase - LiP) using 0.05% guaiacol as a substrate. The highest abundance of decomposer population was found in the block 4 with 9.9 x 106 CFU ml-1, and the lowest in block 6 with 1.9 x 106 CFU ml-1 density. Three superior decomposer isolates were obtained, namely KRB 3.1 (from block area 3), KRB 4.6 (from block area 4), KRB 6.7 isolate (from block area 7). The KRB 6.7 isolate has the highest Lac and MnP enzyme activities, as the following 24.10 U ml-1 and 186.3 U ml-1, while KRB 3.1 isolate from block area 3 has the highest LiP activity with the value 598.57 U ml-1. According to the molecular analysis, those candidate isolates of superior decomposers were identified as Coriolopsis hainanensis (KRB 3.1 isolate), Polyporus thailandensis (KRB 4.6 isolate), and Cerrena aurantiopora (KRB 6.7 isolate). Those are potentially used as the decomposers in solid organic waste fermentation.