Abstract
The isolation from cells of particles such as nuclei, mitochondria, microsomes and chloroplasts is important in biochemical and physiological studies. Cell particles are separated according to size, density and form. Experiments showed that surface properties of particles could determine the fractionation in a liquid-liquid two phase system, complementing conventional fractionation techniques. Aqueous polymer two phase systems were used in preference to organic solvent-water systems. Enzymes and viruses may be distributed in these systems without loss of activity. The usefulness of the method is demonstrated with examples. Other properties of polymer two phase systems are also discussed.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call