Formulation and Effectivity of Noni Fruit (Morinda citrifolia) and Aloe vera Extract Gel (GEMOCA) for Burn Wounds in White Mouse (Mus musculus)

Abstract

Burn wound is an anatomical defect on the skin that occurs due to contact with high-temperature objects. Noni fruit and Aloe vera are known to have various therapeutical activities, including burn wound healing. The research aims to determine the formulation and effects of noni fruit (Morinda citrifolia) and Aloe vera extract gel (GEMOCA) for burn wounds in white mice (Mus musculus). The method of this research was quantitative. Fifteen (n=15) male mice, weighing 40-50 grams were divided into five groups (three treatment groups and two control groups), which consisted of three mice for each group. The burn wounds were made by shaving a 3cm wide area on the back using a 20mm heated metal plate for three seconds. Three treatment groups were treated with Morinda citrifolia and Aloe vera extract (GEMOCA) gel with the following distinct concentration: 1% : 0.5% (F1), 3% : 0.5% (F2), and 5% : 0.5% (F3). Positive control (KP) group was administered with Bioplancenton® gel, whilst negative control (KN) received no treatment. All treatments were conducted every 6 hours for 15 days. Burn wound observation was carried out on day 1, 4, 8 and 15 by measuring the diameter of the wound. The obtained data were analyzed using one-way ANOVA. The result showed that the burn wound diameter in white mice applied with GEMOCA gel in F3 (4.04 cm) had no significant difference with KP group (3.94 cm) and was the fastest recovery time in wound healing among the other treatment groups. This signifies that GEMOCA gel could be an alternative treatment for burn wound healing. The lower result of wound diameter in treatment group compared to positive control group is presumably because of the low active agent content due to manual method for extraction. Therefore, further studies to find a better extraction method to obtain higher concentration of active agent were needed.