The influence of noni fruit (Morinda citrifolia) extract toward the level of SGOT and SGPT enzymes on white mice induced by carbon tetrachloride

Abstract

Surya DH, Mariyah Y, Tahono. 2009. The influence of noni fruit (Morinda citrifolia) extract toward the level of SGOT and SGPT enzymes on white mice induced by carbon tetrachloride. Biofarmasi 7: 87-93. Noni fruit (Morinda citrifolia L.) is a wellknown crop in the society. It is determined for its contents including proxeronine and some antioxidants, i.e. ascorbic acid and beta-carotene that function to maintain and improve cell function. This research used hepatic cells considering the vital function of the hepatic organ in the body. The purpose of this research was to determine the effect of noni fruit extract to reduce hepatic cells damage induced by CCl4. This research was included into laboratory experimental research and used a completely randomized design. The samples consisted of 25 male white mice (Mus musculus) type Swiss Webster with the age between 3-4 months and the weight between 20-30 grams, and divided into 5 groups. The first group was the CCl4 control group, in which white mice were given toxic dosage of CCl4 11 mg/20 g BW without noni fruit extract treatment. The second group consisted of white mice given by noni fruit extract in dosage of 0.56 g/20 g BW for 8 days, and in 8th day they were given by a toxic dosage of CCl4 11 mg/20 g BW. The third group consisted of white mice given by noni fruit extract in dosage of 1.12 g/20 g BW for 8 days, and in 8th day they were given by a toxic dosage of CCl4 11 mg/20 g BW. The fourth group consisted of white mice given by noni fruit extract in dosage of 2.24 g/20 g BW for 8 days and in 8th day they were given by a toxic dosage of CCl4 11 mg/20 g BW. The fifth group consisted of white mice given only water and daily food for 8 days. Blood samples from all white mice were taken after 24 hours to determine the level of SGOT and SGPT enzymes. The results were analyzed by using One-Way Anova statistical test, which continued with Post Hoc Test and Tukey Test. The result of research showed that noni fruit extract in dosage of 0.56, 1.12, and 2.24 g/20 g BW given per oral could reduce SGOT level in 214.48±48.804 U/I, 151.16±22.811 U/I, and 169.62±44.891 U/l, respectively, compared with a positive control of CCl4 that was 296.62±59.254 U/I. Meanwhile, SGPT level became 55.42±4.292, 54.34±6.896, 58.58±8.210 U/I, compared with a positive control of CCl4 that was 83.96±2.931 U/I.