Abstract
Formation of oligomeric Col E 1 DNA is observed in the temperature-sensitive replication mutant Escherichia coli ts CR 34 43 (Col E 1) at the restrictive temperature. Double-labeling experiments indicate that the formation of complex Col E 1 DNA occurs by a replication mechanism and not by a random recombination process involving all of the preexisting circular monomer Col E 1 DNA molecules. Synthesis of complex circular forms of Col E 1 DNA takes place equally well in two different recombination-deficient mutants carrying the same temperature-sensitive replication marker as E. coli ts CR 34 43 .
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