Abstract
Summary: The formation of protoplasts from Geotrichum candidum, by means of a lytic enzyme complex of Streptomyces satsumaensis, was considerably stimulated by adding thiols, including dithiothreitol, 2-mercaptoethanol and cysteamine, to the incubation medium. Treatment of the mycelium with these compounds before incubation with lytic enzymealso enhanced protoplast formation, dithiothreitol giving the most rapid release of protoplasts. The same stimulating effect could be obtained with a proteolytic enzyme. These results suggest the presence of proteinaceous material in the outer layers of the hyphal wall. Geotrichum candidum protoplasts regenerated in liquid as well as solid media if a suitable osmotic stabilizer was present. Up to 20% of the protoplasts could grow into new mycelium.
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