Abstract
SUMMARY: Protoplasts of a strain of Pythium (prl 2142) could be obtained by incubating the mycelium with a combination of helicase and cellulase in the presence of an osmotic stabilizer. This process was inhibited when organic compounds were used as osmotic stabilizers but stimulated by pretreatment of the mycelium with a detergent. The most effective detergent was Triton X100. Regeneration of protoplasts could be demonstrated in liquid or in solid media, but less than 30% of the protoplasts were able to grow into new mycelium. In contrast to protoplast formation, regeneration was inhibited by the inorganic salts used as osmotic stabilizer. The results suggest that the hyphal wall of Pythium is covered with a protective layer of lipid material.
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