Abstract
Conditions are reported for efficient protoplast formation and regeneration in three strains of Streptoverticillium. Mid-exponential phase mycelia, grown in Tryptic-soy broth supplemented with 1.5% (w/v) glycine, were highly sensitive to lysozyme, but the protoplasts did not regenerate. The presence of Ca +2 in protoplast formation media was required for cell regeneration. Mg +2 improved the regeneration yield only in thecase of Streptoverticillium griseocarneum NCIMB 40447. Protoplasts only regenerated in media containing complex nitrogen and carbon sources. Maximum cell regeneration was achieved with protoplasts obtained from mid-exponential growth phase mycelia and plated on an undehydrated Bennet agar overlayered with the same medium containing a lower agar concentration. Sucrose proved to be the optimal osmotic stabilizer and was added to the media for protoplast formation and regeneration.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
