Abstract
Understanding the molecular and biochemical basis of egg development is a central topic in mosquito reproductive biology. Lipids are a major source of energy and building blocks for the developing ovarian follicles. Ultra-High Resolution Mass Spectrometry (UHRMS) combined with in vivo metabolic labeling of follicle lipids with deuterated water (2H2O) can provide unequivocal identification of de novo lipid species during ovarian development. In the present study, we followed de novo triglyceride (TG) dynamics during the ovarian previtellogenic (PVG) stage (2–7 days post-eclosion) of female adult Aedes aegypti. The incorporation of stable isotopes from the diet was evaluated using liquid chromatography (LC) in tandem with the high accuracy (< 0.3 ppm) and high mass resolution (over 1 M) of a 14.5 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (14.5 T FT-ICR MS) equipped with hexapolar detection. LC-UHRMS provides effective lipid class separation and chemical formula identification based on the isotopic fine structure. The monitoring of stable isotope incorporation into de novo incorporated TGs suggests that ovarian lipids are consumed or recycled during the PVG stage, with variable time dynamics. These results provide further evidence of the complexity of the molecular mechanism of follicular lipid dynamics during oogenesis in mosquitoes.
Highlights
Understanding the molecular and biochemical basis of egg development is a central topic in mosquito reproductive biology
The biosynthesis of lipids begins in the midgut; sugars are used as precursors for fatty acid (FA) and triglycerides (TG) s ynthesis[21,22]
Stable isotopes from deuterated water were incorporated into the mosquito sugar diet, and TGs were detected using liquid chromatography coupled to Ultrahigh Resolution Mass Spectrometry
Summary
Understanding the molecular and biochemical basis of egg development is a central topic in mosquito reproductive biology. The monitoring of stable isotope incorporation into de novo incorporated TGs suggests that ovarian lipids are consumed or recycled during the PVG stage, with variable time dynamics. These results provide further evidence of the complexity of the molecular mechanism of follicular lipid dynamics during oogenesis in mosquitoes. Lipids and glycogen are the primary energy reserves for egg development during the immature stages[5,10,11] These teneral reserves are partially consumed during the PVG period; nectar-feeding adds critical reserves during the ORS, and a blood meal triggers VG8,11–14. Their relative abundances and degree of stable isotope incorporation (e.g., deuterium) were measured as a function of the time after adult eclosion
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
