Foliar spraying with bacterial biocontrol agents for the control of common bacterial blight of bean

Maurício Sangiogo,Bianca Obes Corrêa,Johan Manuel Murcia Bermudez,Renata Moccellin,Daniela Pimentel Rodriguez,Andrea Bittencourt Moura

doi:10.1590/s0100-204x2018001000003

Maurício Sangiogo, Bianca Obes Corrêa + Show 4 more

Open Access

https://doi.org/10.1590/s0100-204x2018001000003

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Abstract

Abstract: The objective of this work was to evaluate the effect of foliar spraying with bacterial biocontrol agents (BBAs) on the control of common bacterial blight (CBB) of bean, and on the induction of systemic resistance in bean plants. CBB control by BBAs was evaluated by spraying bean leaves 48 and 24 hours before and after pathogen inoculation (BPI and API, respectively), with: DFs93, Bacillus cereus; DFs513, Pseudomonas veronii; DFs769, B. cereus; the C01 combination, DFs93 + DFs769 + DFs831 (Pseudomonas fluorescens); the C03 combination, DFs348 (Bacillus sp.) + DFs769 + DFs831; and water (control). Systemic effects were analyzed after spraying DFs513, DFs769, C03, and water 72 and 48 hours BPI. Phaseolin production induced by DFs348, DFs513, DFs769, DFs831, and water was also assessed. DFs513, DFs769, and C03 significantly reduced disease incidence (area under disease progress curve), regardless of spraying time and disease severity when sprayed 72 and 48 hours BPI. The DFs769 and DFs831 isolates induced the accumulation of phytoalexin (phaseolin). Therefore, DFs513, DFs769, and C03 show potential for the biocontrol of CBB when applied preventively on bean leaves, besides inducing systemic resistance.

Highlights

Common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli (Smith) (Xap), is considered one of the most important diseases of the common bean (Phaseolus vulgaris L.) crop and the main one among the bacterial group (Bianchini et al, 2005)
The objective of this work was to evaluate the effect of foliar spraying with bacterial biocontrol agents (BBAs) on the control of common bacterial blight (CBB) of bean, and on the induction of systemic resistance in bean plants
BBAs significantly affected the severity of CBB symptoms, but not the incidence of the disease

Summary

Introduction

Common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli (Smith) (Xap), is considered one of the most important diseases of the common bean (Phaseolus vulgaris L.) crop and the main one among the bacterial group (Bianchini et al, 2005). Common bacterial blight, caused by Xanthomonas axonopodis pv. The control of common bacterial blight is based on preventive measures (Moura et al, 2009). Several authors have reported the biological control of bacterial diseases caused by Xanthomonas spp. Bacillus and Pseudomonas, are known for their efficiency and diversity in producing antibiotics responsible for the effective control of various diseases (Khabbaz et al, 2015); these two genera harbor systemic resistance-inducing species (Akram et al, 2015; Planchamp et al, 2015). The diverse modes of action of the bacterial biocontrol agents attract attention to their preventive and curative uses, inducing resistance and acting by antibiosis, respectively

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