FNIP-1 Expression in Mice Muscle Negatively Correlated with Myoglobin Level and Endurance Performance on Treadmill Run Test

Abstract

Previously, FNIP-1 was found to play a critical role in the coupling of mitochondria function to restore ATP. Mitochondria was need to be supplied sufficient oxygen by myoglobin in the myocyte. We proposed that FNIP-1 also regulated myoglobin levels to support endurance performance. This study aimed to investigate whether FNIP-1 expression in muscle was correlated with myoglobin level and endurance performance on the treadmill run test. Male adult balb/c mice performed a run on the treadmill for as long as possible. The duration of running was measured as endurance performance. Its calf muscles were analyzed for myoglobin level and FNIP-1 expression. Lower FNIP-1 expression in calf muscle was found on higher levels of myoglobin and the longer duration of running on a treadmill. The Pearson test showed that the P value was <0.05, which indicated that there was a correlation between FNIP-1 and myoglobin with a score of -0.77, which indicated that there was a negative correlation. The Spearman test also showed that the P value was <0.05, which indicated that there was a correlation between FNIP-1 and endurance performance with a score of -0.70, which indicated that there was a negative correlation. So, FNIP-1 expression negatively correlated with myoglobin level and endurance performance of mice. Further study needs to investigate the FNIP-1 expression in human muscle to look at its correlation with the level of lactate, oxygen saturation (SaO2), oxygen fraction (FiO2), and maximum volume of oxygen uptake (VO2 max). Keywords: FNIP-1, muscle, endurance, myoglobin, healthy lifestyle.