Abstract
The fluorescence properties of acriflavine and methylbenz( a)anthracene conjugates of DNA and several biosynthetic polyribonucleotides have been examined and compared. Both types of label respond in a qualitatively similar way to helix → coil transitions involving the polynucleotides. While the terminal-labeled acriflavine conjugates showed only a single fluorescence decay time, the methylbenz( a)anthracene conjugates gave decay curves which could best be fitted on the assumption of two fluorescent species, with different decay times. When labeled poly(rA) was incorporated into the rA : rU bihelical complex the extent to which rotational mobility, as measured by polarization, was lost at the early stages of complex formation differed for the two labels.
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