Abstract
Quality control of drug fleroxacin dosage, its monitoring in biological fluids, and research of drug’s metabolism and action are an important analytical task. A new chemiluminescence (CL) reaction system was established for the determination of fleroxacin (FLX). The trivalence dysprosium-sensitized CL emission mechanism was investigated by comparing the fluorescence emission with CL spectra. The CL spectra of FLX-KMnO 4 -Na 2 S 2 O 3 -H 6 P 4 O 13 system are from the narrow characteristic emission of Dy 3+ at 482 and 578 nm ( 4 F 9 → 6 H 15/2 , 4 F 9 → 6 H 13/2 ) through the energy transfer from the excited SO 2 * to analyte, followed by intramolecular energy transfer from analyte* to Dy 3+ . The optimum conditions for CL emission were investigated and optimized. The relationships between the relative CL intensity and the concentration of the studied analyte have good linearity. The detection limit for FLX was 3.0×10 -10 g/mL. The relative standard deviation is 2.0% for 11 determinations of FLX at 2.0×10 -6 g/mL. The proposed CL system has been successfully applied for the determination of FLX in the injections and urine sample with satisfactory result. .
Highlights
Fleroxacin; PharmaceuticalFleroxacin [FLX, 6,8-difluoro-1-(2-fluoroethyl)-1,4-dihydro-7-(4methyl-1- piperazinyl)-4-oxo-3-quinoline carboxylic acid] is a new fluoroquinolone antibiotic that exhibits strong bactericidal activity against a wide range of Gram-negative and Gram-positive bacteria [1]
High-performance liquid chromatography (HPLC) with fluorescence detection has been developed for the measurement of FLX in rat plasma using a solid-phase extraction column [11], and FLX in serum [12]
Capillary electrophoresis (CE) and HPLC have the advantage of high separation capability suitable for components determination, and disadvantage of lower sensitivity
Summary
Fleroxacin; PharmaceuticalFleroxacin [FLX, 6,8-difluoro-1-(2-fluoroethyl)-1,4-dihydro-7-(4methyl-1- piperazinyl)-4-oxo-3-quinoline carboxylic acid] is a new fluoroquinolone antibiotic that exhibits strong bactericidal activity against a wide range of Gram-negative and Gram-positive bacteria [1]. Quality control of drug dosage, its monitoring in biological fluids, and research of drug’s metabolism and action are an important analytical task. It is necessary to establish sensitive analytical technique. Several methods have been described for the determination of FLX either in pure form, in dosage forms or in biological fluids [3,4,5,6,7,8,9,10]. High-performance liquid chromatography (HPLC) with fluorescence detection has been developed for the measurement of FLX in rat plasma using a solid-phase extraction column [11], and FLX in serum [12]. Capillary electrophoresis (CE) and HPLC have the advantage of high separation capability suitable for components determination, and disadvantage of lower sensitivity
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