A novel sensitized chemiluminescence flow injection system for the determination of adriamycin and mitomycin

Abstract

A novel chemiluminescence (CL) method was established for two anticancer drugs, adriamycin (ADM) and mitomycin (MMC), based on potassium permanganate oxidation in the presence of formaldehyde. The sensitized CL emission mechanism was developed by comparing the fluorescence emission with CL spectra. Illuminant was the singlet state bi-molecule oxygen, 1O21O2 (1Δg1Δg), from 1O2 (1Δg) which was produced in the reaction system, and emitted CL spectra at 639 nm or 649 nm. The presence of formaldehyde may accelerate the generation of 1O2 (1Δg) and sensitized CL emission. The optimum conditions for CL emission were investigated and optimized. The relationships between the relative CL intensity and the concentration of the studied analytes found to be linear. The detection limit was 3 × 10−8 g ml−1 for ADM and 3 × 10−9 g ml−1 for MMC. The relative standard deviations are 2.2% and 1.8% for determinations of ADM at 2.0 × 10−6 g ml−1 and MMC at 2.0 × 10−7 g ml−1, respectively. The proposed sensitized CL system was successfully applied to the determination of ADM and MMC in their injections with satisfactory results.