Fingerprint Analysis of Herbal Raw Materials available in Market Belongs to Combretacea And Fabaceae family by HPTLC technique using antioxidant markers

Abstract

The prime aim of the study is to notice the flavonoids, phenolic acids and xanthones in five commercial herbal raw materials namely Pterocarpum marsupium Bark, Ormocarpum cochinchinense leaf, Indigofera tinctoria leaf, Terminalia arjuna bark and Terminalia chebula fruit obtained from Fabaceae and Combretacea family used in daily domestic needs to confirm the presence of common antioxidant secondary metabolites in herbal raw materials. Results of the study clearly revealed that these raw materials from Fabaceae and Combretacea family contains flavonoids, phenolic acids and xanthones. The developed simultaneous HPTLC method can be employed for the routine investigations of flavonoids, phenolic acids and xanthones in herbal raw materials. Pterocarpum marsupium Bark, and Ormocarpum cochinchinense leaf, was ascertain the presence of Rutin and reveals 0.006%,0.051% respectively. Gallic acid was only orginate in Pterocarpum marsupium Bark of about 0.113%. Quercetin was found to be 0.061%, 0.239%, 0.336%,0.066% and 0.036% respectively in all tested five extracts. Catechin was found to be 0.203% in Terminalia arjuna bark. Mangiferin was found to be 0.009%. in Indigofera tinctoria leaf.