Fibroblast test cells of embryo of Super Java Chicken as an indicator to test toxicity and malignancy

Abstract

Fibroblast is one component of connective tissue cells in polygonal or stellate with cytoplasmic processes or projections. In vitro, tissue culture is an excellent medium used in biomedical research. The goal of this study was to analyze Toxicity and Malignancy in Embryo of Super Java Chicken as Potential Candidates of Strong Poultry in Indonesia. This study used Preparation of fibroblast primary cell culture of Java super chicken embryos, Pathogenicity test using fibroblast cells from Super Java chicken, Toxicity test using fibroblast cells from Super Java Chicken. Primary fibroblast cell culture of Java super chicken embryos was prepared from 10-day-old brood chicken eggs free of pathogens. Cells were prepared in 96-well microplates with minimal essential medium (MEM) containing 10 % fetal calf serum (FCS) 100 IU/ug penicillin/streptomycinVirus isolates were diluted in stages from 10-2 to 10-5 and inoculated into Java Super Chicken Fibroblast cell culture. The result showed that the negative control of the samples had a faster proliferative power than the fibroblast cell culture of Java super chicken, which was treated with concentrations of 10-2; 10-3; 10-4; 10-5. Moreover, before being inoculated with the virus, the confluent fibroblast cells of Java super chicken looked oval and regular. The day after infection, syncytia (large multinucleated cells) began to form on a small scale and became more pronounced on the second and third post-infection days. CPE was found in the 10-2,10-3 and 10-4 virus dilutions, and CPE was not found in the 10-5 dilution.