Abstract
The propagation of infectious laryngotracheitis virus (ILTV) has been described using primary cell cultures derived from chicken embryo liver and kidney or embryonated eggs, but these cultures use Specific Pathogen Free (SPF) eggs that are time and cost expensive. Since cell line cultures are easier to maintain in laboratory conditions, the growth of ILTV was evaluated in five different cell cultures: chicken embryo related cells (CER), a cell hybrid derived from chicken embryo fibroblasts cells and BHK-21; Vero, from African green monkey kidney cells; HD11, a chicken macrophage cell line; CEC-32, an avian fibroblast cell line and a primary cell culture of chicken embryo fibroblasts (CEF). Cytophatic effect was observed until 96 hours following inoculation and the detection of the viral DNA was performed by PCR. The HD11 and CEC-32 cell lines did not support the virus growth but CEF and Vero, as already described were permissive cultures for propagation of ILTV. The results also showed that the CER cell line can be used for primary isolation and replication of ILTV.
Highlights
Infectious laryngotracheitis virus (ILTV) is classified as a member of the family Herpesviridae in the subfamily Alphaherpesvirinae
The isolation of ILTV from field material has been described using embryonated chicken eggs inoculated in the chorioallantoic membrane (CAM) [12], and primary cell cultures derived from chicken embryo liver (CEL), chicken embryo kidney (CEK), chicken embryo fibroblast (CEF) and a cell line derived from an African green monkey kidney (Vero) [10]
The cells tested were a primary cell culture of chicken embryo fibroblasts (CEF) [12], that was compared with the cell lines: chicken embryo related cells (CER), a cell hybrid derived from chicken embryo fibroblasts cells and BHK-21 [19], a chicken fibroblast cell line (HD11) [3], a quail fibroblast cell culture, CEC-32 [23] and a mammalian cell line derived from African green monkey kidney (Vero) [12]
Summary
Infectious laryngotracheitis virus (ILTV) is classified as a member of the family Herpesviridae in the subfamily Alphaherpesvirinae. The isolation of ILTV from field material has been described using embryonated chicken eggs inoculated in the chorioallantoic membrane (CAM) [12], and primary cell cultures derived from chicken embryo liver (CEL), chicken embryo kidney (CEK), chicken embryo fibroblast (CEF) and a cell line derived from an African green monkey kidney (Vero) [10]. Due to the short life span, cost and the time spent to produce primary cell cultures, an option to enhance the number of viral particles can be the use of a continuous cell line. Such cell lines are especially relevant to many laboratory procedures that require high virus titers, such as antigen production for diagnostic tests, animal inoculation experiments and vaccine production. The purpose of the present study was to compare the susceptibility of different cell lines for ILTV propagation
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
