Abstract
Pectins have been isolated from an ethanol-insoluble residue of sugar-beet pulp by sequential extraction with water, oxalate, hot dilute acid, andcold dilute alkali in yields of 2.2, 0.53, 20, and 11%, respectively. They were purified by chromatography on DEAE-cellulose at pH 4.8,or by precipitation with copper sulphate (alkali-soluble pectin). The pectins had fairly low molecular weights, a high degree of acetylation, and relatively high contents of neutral sugars, but there were clear differences beteen the four fractions. The main neutral sugars in each pectin were arabinose and galactose, and rhamnose, fucose, xylose, mannose, and glucose were also present. The fractions were homogeneous in ion-exchange and gel-filtration chromatography. Polyphenols (1–2%) and possibly proteins (3–6%) were associated with the purified pectins. In addition, feruloyl groups (up to 0.6%) were linked mainly to the acid-soluble and alkali-soluble pectins.
Published Version
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