Abstract
Introduction: Female infertility is a condition that is currently treated through the maximization of existing reserves; a necessity due to the fact that a true reversal of the processes leading to infertility is not yet technologically possible. This experiment examined the ability of mesenchymal stem cells to differentiate into primordial germ cells (PGC) when cultured onto the placental scaffold. Methods: To produce the scaffolds, the cotelydons were collected and decellularized by umbilical vessel SDS perfusion. Adipose derived cells were isolated based on rapid adherence to the plastic. Results: The isolated cells displayed the markers CD90 and 105, while lacked CD34 and CD45. When seeded onto the scaffold, the cells successfully differentiated into PGC like cells, displaying typical PGCs markers, including STELLA, OCT4, DAZL and VASA. Discussion: These materials were chosen for their low cost and wide availability. The data in this study show the promising potential of cell-scaffold complex to support the development of female tissue engineering-based regenerative medicine therapies.
Highlights
Female infertility is a condition that is currently treated through the maximization of existing reserves; a necessity due to the fact that a true reversal of the processes leading to infertility is not yet technologically possible
Mesenchymal stem cells represent a promising cell source to be used in the recellularization process, because they do not form tumors when injected in vivo and because they are easy to obtain and differentiate, exert immunomodulatory effects, and are able to modulate angiogenesis, cell proliferation, and apoptosis [31,32]. as such, the aim of this study is to investigate the potential of decellularized placenta to be recellularized with adipose tissue-derived cells for further primordial germ cell differentiation
The pellet was transferred into a T25 culture flask (Corning, New York, USA), and the medium was changed three hours after the beginning of the culture, a protocol that has been previously described for the selection of a mesenchymal stem cell population at the first passage [34,35]
Summary
Female infertility is a condition that is currently treated through the maximization of existing reserves; a necessity due to the fact that a true reversal of the processes leading to infertility is not yet technologically possible. This experiment examined the ability of mesenchymal stem cells to differentiate into primordial germ cells (PGC) when cultured onto the placental scaffold. Systematic infertility is a pressing concern in agriculture, where it currently poses a threat to global livestock supply chains, that of the dairy industry [3] The importance of this problem in both bovine species and humans allows for a doubly beneficial translational investigation. Cows possess strong similarities to humans in regard to their reproductive system, which extend to the areas of physiology, folliculogenesis, oocyte development [5], the oocyte selection processes, and age-associated endocrinal events, among among other areas [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Current Trends in Biomedical Engineering & Biosciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
