FEATURES OF THE ULTRAMICROSCOPIC STRUCTURE OF THE SPLEEN IN THE RATS OF THE DIFFERENT PERIODS OF POSTNATAL ONTOGENESIS AT THE EXPERIMENTAL IMMUNOSUPRESSION

Abstract

Aim of research is to study the features of ultramicroscopic structure of the white pulp of the spleen in the rats at the different stages of postnatal ontogenesis after introduction of immunosuppressive drug сyclophosphamide. 180 white noninbred male rats of three age periods: puberty (1 st group), reproductive (2 nd group) and period of the expressed senile changes (3 rd group) served as a material for investigation. Сyclophosphamide was injected in a dosage resulting in immune depression (200 mg/kg of body weight of animal). Study of ultrastructural characteristics of white pulp of the spleen was performed on ultrathin sections with electron microscope. It was established that introduction of cyclophosphamide results to morphological alteration of spleen of white rats as a hypoplasia of the white pulp. The highest level of reactivity of the spleen in reply to introduction of cytostatic drug is observed at the animals of the 1 st group. Сhanges of morphological characteristics of the white pulp of the spleen in the rats of the 2 nd and the 3 rd groups take place on a background of the evolutional changes of organ. In the animals of the 2 nd and the 3 rd groups after the cyclophosphamide administration high activity of apoptotic processes in the white pulp of the spleen induced by cytostatic drug occurring against the backdrop of involutive changes in the organ is observed.