FEATURES OF THE TOPOGRAPHY AND NUMBER OF SBA+-В-LYMPHOCYTES IN THE MESENTERY OF THE INTESTINE IN NORMAL CONDITIONS AND DURING THE FORMATION OF THE ADHESION PROCESS

Abstract

The study of the structural organization of the peritoneum as an immunocompetent organ will contribute to the solution of practical tasks, namely factors that control immune local and systemic reactions in the body. The question of the presence of these cells and their topography by the method of lectin histochemistry in the tissues of the peritoneum at the time of its formation, the peculiarities of the structure in different periods of onotogenesis in the norm and under the influence of endo- and exogenous factors remains not fully investigated. Carbohydrate specificity is used as a criterion for the functional classification of lectin receptors on the surface of cells, which indicates the processes of adhesion to various molecules, including fibrin and immune complexes. The aim of our study was to determine the peculiarities of the topography and the number of B-lymphocytes of the intestinal mesentery in rats in normal conditions and during the adhesion process with the help of soy lectin (SBA). The process of adhesion formation in rats of the II group was simulated by a single intraperitoneal injection of 0.5 ml of a 20% talc suspension into the area pelvis according to the method of Volyanska O.G. (2013). Animals were removed from the experiment 7, 14 and 21 days after the injection. Detection of lymphocytes that differ phenotypically according to carbohydrate residues was performed using soy lectin (SBA). The number of immunocompetent cells per standard area of 1000 μm2) was calculated.The morphology, topography and number of SBA+-lymphocytes, identified as B-lymphocytes, in the intestinal mesentery, as one of the derivatives of the peritoneum, are described. An increase in the number of SBA+ lymphocytes indicates the activation of the nonspecific humoral link of immunity, which is associated with excessive deposition of fibrinoid and affects the morphofunctional state of the peritoneum and its derivatives. Probably, lymphoid tissue associated with serous membranes (SALC) gives rise to the origin of B1-lymphocytes and is the place of their constant renewal. The leakage of fibrin leads to the formation of fibrin plaques, which are pathogenetically capable of changing the structural barrier between the connective tissue and the vessels of the peritoneum, which at the moment remains insufficiently studied. Quantitative studies of the accumulation of fibrin layers by the method of lectin histochemistry are planned.