Abstract
Aim.To study the ionic mechanisms which underlie regulation contractile activity of vascular smooth muscles and the erythrocytes membrane potential from spontaneously hypertensive rats.Materials and methods.The effect of acetylcholine (ACX), 10 μM, and Ca2+ -ionophore A23187, 10 μM, on the contractile reactions of isolated smooth muscle segments of the aorta from 11-week-old spontaneously hypertensive rats (SHR) and Wistar – Kyoto rats (WKY) induced by phenylephrine (PE), 1 μM, was measured by the mechanography. 11-week-old SHR were divided into control and experimental groups depending on the drug administration (amlodipine 10 mg/kg given intragastrically). Blood pressure (BP) in SHR was measured before and after treatment. The amplitude of the hyperpolarizating response (HO) and the activity of Ca2+-dependent K+-channels of the erythrocyte membrane of SHR were performed with potentiometric method.Results.In response to the stimulating effect of the ACX or the calcium ionophore A23187, the smooth muscle segments of the aorta from the WKY and SHR precontracted with PE were relaxed. To the addition of the ACX, the vascular segments of the SHR responded with a stronger dilatation than the WKY segments, but not the action of the Ca2+-ionophore. Treatment of SHR with blocker of Ca2+-channels of L-type amlodipine caused a decrease the BP, and an increase in the relaxing effect of intact aortic segments on the ACX and Ca2+-ionophore. There was an increase the amplitude of HO and activity of Ca2+-dependent K+-channels of the erythrocyte membrane from SHR.Conclusion.Relaxation of vascular smooth muscle in SHR is caused by changes in the endothelial function and Ca2+-dependent ionic conductivity of the myocyte membrane. An increase of the hyperpolarizating response of the erythrocyte membrane from rats with spontaneous hypertension is associated with activation of potassium channels. The selective effect on these intracellular targets can serve as a basis for the development of antihypertensive drugs.
Highlights
CONFORMITY WITH THECPORNINFOCIRPMLEITSYOWF EITTHHICS The study TwHaEs aPpRpIrNoCveIdPLbEyStOheF lEoTcaHlICetShics committee undTerheSSstMudUy.s committee under Siberian State Medical University (SSMU) (Protocol No 4995 оf 27.10.2016)
Дилатационные реакции сосудистых гладких мышц крыс SHR обусловлены изменением эндотелиальной функции и Са2+-зависимой ионной проводимости мембраны миоцитов
STheaslmyanEaleevnaASl.e,xSatnuddeerntY, uM.,edPihcoD-,BJioulnoiogircaSlcFieanctuisltyR, eSsSeMarUch, eTro, mLsakb,oRruatsosirayn oFfedBelroaotidonC. irculation Pharmacology, GRIPR, TNRSMhaCm,aRnAaeSv, TAolmexsakn, dReurssYiaun.,FePdheDr,atJiuoni.oOr RSCcIieDntiiDst0R00e0se-a0r0c0h3e-2r,34L8a-b14o4ra4t.ory of Blood Circulation Pharmacology, GRIPR, TNRPMusCh,kRinAaSE,kTaotemrisnka, RVu.,ssRiaensidFeendteDraotciotonr.,ODRepCaIrDtmiDen0t0o0f0R-0a0d0io3d-2ia3g4n8o-1si4s4a4n.d Radiotherapy, Siberian State Medical University (SSMU), Tomsk, Russian Federation
Summary
Для цитирования: Носарев А.В., Бирулина Ю.Г., Петрова И.В., Ковалев И.В., Гусакова С.В., Смаглий Л.В., Тесля Е.С., Шаманаев А.Ю., Пушкина Е.В., Медведев М.А. Изучение ионных механизмов регуляции сократительной активности сосудистых гладких мышц и мембранного потенциала эритроцитов у спонтанно-гипертензивных крыс. Механографическим методом исследовано действие ацетилхолина (АЦХ), 10 мкМ, и Са2+-ионофора A23187, 10 мкМ, на контрактильные реакции изолированных гладкомышечных сегментов аорты 11-недельных спонтанно-гипертензивных крыс (spontaneously hypertensive rats, SHR) и крыс Wistar – Kyoto (WKY), индуцированные фенилэфрином (ФЭ), 1 мкМ. У крыс SHR отмечено повышение амплитуды ГО активности Са2+-зависимых К+-каналов мембраны эритроцитов. Дилатационные реакции сосудистых гладких мышц крыс SHR обусловлены изменением эндотелиальной функции и Са2+-зависимой ионной проводимости мембраны миоцитов. Цель данной работы – изучение ионных механизмов регуляции сократительной активности сосудистых гладких мышц и мембранного потенциала эритроцитов у спонтанно-гипертензивных крыс
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