FEATURES OF BILE-FORMING FUNCTION OF THE LIVER IN WHITE RATS IN RESECTION OF DIFFERENT VOLUMES OF ITS PARENCHYMA

Abstract

Objective - to study the peculiarities of bile-forming function of the liver in resection of various volumes of its parenchyma.Material and methods. The research was conducted on 60 male Wistar rats, which were divided into 4 groups. Group 1 consisted of 15 intact animals, group 2 - 15 rats after resection (31.5%) of its parenchyma; group 3 was composed of 15 animals after removal of the liver volume (42.0%), group 4 - 15 rats following the resection (58.1 %) of its parenchyma. In a month after the beginning of the experiment bile- excreting function was studied in rats under thiopental anesthesia by means of catheterization of the common bile duct and bile taking for 1 hour. Euthanasia of experimental animals was carried out via bloodletting under thiopental anesthesia in a month from the beginning of the experiment. Histologic micronutrients of the liver were studied light-optically. Quantitative indicators were processed statistically.Results. The intensity of bile secretion wad detected to be decreased 7.4 % compared to the control, the content of total bilirubin in bile - 9.7 %, direct bilirubin - 12.6 %, concentration of bile acids in bile - 5.5 %, cholesterol - 6.7 %, trypsin - 9.3%, alkaline phosphatase - 8.3%, acid phosphatase - 8.4%, creatine phosphokinase - 9.3% in a month following the resection (42.5%) of the liver parenchema. After the removal of 58.1 % of the liver parenchyma, the investigated indexes of bile formation and bile secretion were more pronounced in comparison with the previous group.Light-optically a plethora of venous vessels, areas of hepatocytes hypertrophy and dystrophy, the appearance of lymphoid-hystiocytic infiltrates and increased stromal structures in micropreparations of the liver of 3rd and 4th groups were observed. The revealed morphological changes dominated in resection of 58.1 % of the liver parenchyma.Conclusions. Resection of 42.0% and more of the volume of the liver parenchyma in white rats leads to disturbances of bile-forming and bile excretion functions of the liver, which is characterized by a decrease in the intensity of its secretion, decrease in the amount of bile acids, cholesterol and enzymes in the bile, increase in lithogenic properties.