Fe3O4@Au core satellite magnetic nanoparticles to enhance colorimetric immunosensor response

Abstract

A newly developed colorimetric immunosensor based on gold-coated magnetic nanoparticles (Fe3O4@Au) is presented, and its application for the detection of human immunoglobulin G (IgG) in water is demonstrated. By taking advantage of both the localized surface plasmon resonance (LSPR) phenomenon of gold nanoparticles and the magnetic property of the core, the Fe3O4@Au immunosensor provides a fast and effective method for detecting analytes. In a sandwich scheme, Fe₃O₄@Au nanoparticles are used to enhance the response of the nanostructured gold surface made of gold nanoparticles randomly placed onto a glass coverslip. Specifically, the detection of the target analyte (human IgG) occurs when Fe₃O₄@Au nanoparticles bind to the target from the top in the presence of a magnetic field, leading to a change in the absorption spectrum of the nanostructure. Preliminary results have shown that the colorimetric immunosensor can achieve a limit of detection of 1 ng/mL, with a measurement carried out in only 10 minutes. The use of gold-coated magnetic nanoparticles in conjunction with the plasmonic surface offers great potential for the sensitive and specific detection of analytes. This could pave the way for future applications of the immunosensor in rapid testing and mass screening