Fatty Alcohol-Assisted Electro-Driven Liquid Membrane Multi-Stage Extraction Technology for Selective Separation and Purification of 3,4-Dihydroxybenzaldehyde from Salvia miltiorrhiza Bge.

Abstract

3,4-Dihydroxybenzaldehyde (DHB) is a highly effective water-soluble compound in the traditional Chinese medicine Salvia miltiorrhiza Bge. Which demonstrates clear therapeutic effects against a variety of diseases. Therefore, the development of a selective approach for the separation of 3,4-dihydroxybenzaldehyde is of great significance. In this study, electro-driven extraction combined with multi-stage extraction technology was employed for the first time to separate 3,4-dihydroxybenzaldehyde from S. miltiorrhiza Bge. Decoction (Recovery ≥ 46.09%). Five long-chain fatty alcohols were selected as the supported liquid membrane (SLM) in the electro-driven extraction. Factors influencing extraction efficiency, including pH, voltage, and extraction time, were optimized using a design of experiments. Under the optimal conditions, the linearity of 3,4-dihydroxybenzaldehyde ranged from 0.01 to 100 μg·mL−1), with limits of detection (LOD) and quantification (LOQ) of 5 and 17 ng·mL−1, respectively. The precision of electro-driven liquid membrane extraction for 3,4-dihydroxybenzaldehyde was below 8.42%. This paper reports novel electro-driven liquid membrane extraction for selectively isolating 3,4-dihydroxybenzaldehyde from S. miltiorrhiza Bge.