Extraction of volatile organic compounds from leaves of Ambrosia artemisiifolia L. and Artemisia annua L. by headspace-solid phase micro extraction and simultaneous distillation extraction and analysis by gas chromatography/mass spectrometry

Ok Yeon Song,Joon Ho Hong,Hui Eun Kim,Md Atikul Islam,Ji Young Jeong,Ji Hyeon Son,Naeem Khan,Kyong Su Kim,Nargis Jamila

doi:10.1007/s10068-020-00875-8

Abstract

This study was designed to analyze the volatile organic compounds in the leaves of Ambrosia artemisiifolia L. and Artemisia annua L. from Korea. For extraction of volatile compounds, headspace-solid phase micro extraction (HS-SPME) and simultaneous distillation extraction (SDE) were applied and analyzed by gas chromatography/mass spectrometry (GC/MS). From the results, SDE extraction was found to give the highest concentration of volatile compounds with an average concentration of 1,237.79 mg/kg for A. annua L. leaves compared to 1,122.73 mg/kg by HS-SPME technique. A total of 116 volatile organic compounds were identified, including 76 similar volatile organic compounds detected by both the methods of extraction in leaves of subject species at varying concentrations. Among these 33 volatile organic compounds were reported for the first time from the subject plant species. Thus the present research findings extend the characterization of volatile organic compounds from leaves of A. annua L. and A. artemisiifolia L. species and reported some distinguishing compounds which may be used for their discrimination.

Highlights

Ambrosia artemisiifolia L. leaves are highly allergenic pollens, causing great agricultural losses (Molinaro et al, 2016)
The present research findings extend the characterization of volatile organic compounds from leaves of A. annua L. and A. artemisiifolia L. species and reported some distinguishing compounds which may be used for their discrimination
From literature search it was come to know that headspace-solid phase micro extraction (HS-SPME) and simultaneous distillation extraction (SDE) techniques were not used to isolate volatile organic compounds from A. artemisiifolia L. leaves and there was no comparative study found on the volatile organic compounds of A. artemisiifolia L. leaves and A. annua L. leaves. Both HSSPME and SDE techniques coupled to gas chromatography/mass spectrometry (GC/MS) analysis were applied to establish the comparative results of volatile organic compounds profile of A. artemisiifolia L. leaves and A. annua L. leaves

Summary

Introduction

Ambrosia artemisiifolia L. (common ragweed) leaves are highly allergenic pollens, causing great agricultural losses (Molinaro et al, 2016). Headspace-solid phase micro extraction (HS-SPME) is a quick, solvent less, and simple method for analysis of volatile organic compounds (Holt, 2001; Kataoka et al, 2000; Zhang et al, 1994). This technique is a non-invasive and non-destructive that avoids solvent impurity contamination (Heath and Reineccius, 1986). Both HSSPME and SDE techniques coupled to GC/MS analysis were applied to establish the comparative results of volatile organic compounds profile of A. artemisiifolia L. leaves and A. annua L. leaves. It was attempted to report distinguishing marker volatile organic compounds which may be used for their identification

Materials and methods

Results and discussions

Compliance with ethical standards