Extraction, Isolation Of Chemical Compounds And Evaluation Of Wound Healing Activity Of Bombax Ceiba Plant

Abstract

Herbal plants have a lot of benefits over conventional drugs, including the fact that they usually have less frequent side-effects and might be non-toxic if used for a long time. B. Ceiba is an enormous, stunning, and deciduous tree and native for Northern Australian origin. The present study was based on the extraction, isolation of chemical compounds and evaluation of wound healing activity of Bombax ceiba plant. The chemicals and instruments were obtained from the certified manufacturer and suppliers only. B. ceiba roots and flowers were collected and authenticated from a Botanist at MJPRU Bareilly. The extraction process was done by maceration process. Pharmacological (wound healing) potential was evaluated by wound contraction, re-epithelization, and histopathology. The n-hexane, chloroform, ethyl acetoacetate and methanol extracts of B. ceiba were examined for its phytoconstituents. Wistar rats weighing 130-210g were obtained from the Dept. of Pharmacy, MJP Rohilkhand University Bareilly. The % yield was obtained as 59% for n-hexane, 62% for chloroform, 58% for ethyl acetoacetate and 65% for methanol. According to the preliminary phytochemical analysis, the methanolic B. ceiba extracts showed excellent phytoconstituents of alkaloids, flavonoids, glycosides, carbohydrates, etc. when compared with other solvents extracts. In results, all the groups of animals showed an excellent wound healing activity when compared with control group after 15th day of exposure with treatments. The B. ceiba N-hexane, chloroform, ethyl acetoacetate, and methanol demonstrated rats showed the re-epithelization period of 11.20±0.62**, 12.17±1.30***, 9.3±1.23** and 10.4±1.12***, respectively. It is concluded that each of these has considerably exhibited wound healing potential. It suggests, to isolate the active constituent responsible for the activity.