Evaluation of Antibacterial and Wound Healing Activities of Ethanolic Extract of Linum Usitatissimum (Flax Seed) on Rabbits

Abstract

Aim: Linum usitatissimum was evaluated for its antibacterial and wound-healing capacity.
 Methods: This method involved the collection and authentication of the plant, preparation of herbal extract, phytochemical analysis for the prepared extract, development of an herbal formulation for the prepared extract, evaluation of the antibacterial activity of Linum usitatissimum seed extract and the evaluation of the wound healing activity by Excision wound model. The antibiotic activity was studied by cup plate method using a nutrient agar medium. The zone of inhibition was then determined after culturing and incubation.
 Results: The results of the extract of Linum usitatissimum show antibacterial activity as demonstrated by zone of inhibition when cultured with selected bacterial species (both gram positive and gram negative). Different concentrations of (200 ?g /ml) and (400?g/ml) were used. The extract showed an improved wound healing capacity from the third application as demonstrated by the increase in the percentage of healing (9.55 ± 0.04%) with a significant increase on the sixth day (23 ± 0.4%). Betadine was used as a standard medicament for wound healing.
 Conclusion: The present research study demonstrated that the medicinal plant Linum usitatissimum seed extract possesses significant antibacterial and wound-healing properties. Based on the observation and the significant results obtained, the researchers can draw that the ethanolic seed extract of Linum usitatissimum produced antibacterial and wound healing activities and the results were authenticated by comparing with reference drugs. Further scientific studies can be taken up and detailed study on the preliminary phytochemical constituent present which may be responsible for these biological activities. At this junction of time, this research study provides justification and scientific evidence for further pharmacological activities that are evaluated in our laboratory.