Abstract
In this paper, the corn-stalk cellulose (CSC) was extracted from the corn-stalk pretreated by the immobilized enzyme which was prepared using xylanase and laccase. The immobilization rate was strongly affected by such conditions as the carrier concentration and the carbodiimide (EDC) concentration. The structure of CSC was characterized by Fourier infrared (FTIR). X-ray diffraction (XRD) was used to verify the crystallinity of CSC. The crystallinity of CSC was 74.13%, which was increased by 25.95% than that of the corn-stalk. The morphologies of the corn-stalk pretreated by the immobilized enzyme and the CSC extracted were confirmed by scanning electron microscopy (SEM). The experimental results showed that the cellulose content of the cellulose obtained only by acid–base method was 85.46%, and that of the cellulose obtained by immobilized enzyme was 96.72%. What indicated that the immobilized enzyme pretreatment could loosen the cell well of corn-stalk and partly remove lignin and hemicelluloses from corn-stalk, which was beneficial for the further extraction of cellulose by other treatment methods.
Highlights
Corn is one of the most important crops in the world
X-ray diffraction (XRD) analyses was performed at room temperature using a D8 Bruker in a range of diffraction angles from 5 to 80 at the scanning speed of 2 minÀ1
The reason could be that the more ethyl-3dimethyl amino propyl carbodiimide hydrochloride (EDC) added, the more possible the condensation would be
Summary
Corn is one of the most important crops in the world. In addition to fruits, corn had whiskers, husks, stalks and other byproducts, of which the annual output of stalks was as high as about 350 million tons in China. Mainly including mechanical crushing, steam explosion,[6,7,8] high-energy radiation[9,10] and ultrasonic[11,12,13,14] auxiliary extraction methods, etc., are generally used in cellulose pretreatment process or auxiliary process. Their purpose is to remove lignin, hemicelluloses and other components that have protective actions on cellulose. The immobilized enzymes prepared using xylanase and laccase were used to pretreat stalk for the rst time, and the acid–base method was used to extract cellulose, which greatly improved the effect of cellulose separation and extraction
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