Extract of Microcystis water bloom affects cellular differentiation in filamentous cyanobacterium Trichormus variabilis (Nostocales, Cyanobacteria)

Abstract

Cyanobacteria produce a wide spectrum of biologically active compounds such as microcystins, whose effects on photoautotrophic organisms and role in the aquatic ecosystems have been most intensively discussed and studied. In our study, we examined effects of semipurified Microcystis extract containing microcystins (0.2–20 nM corresponding to 0.2–20 μg L−1) on age-induced cell differentiation of filamentous cyanobacterium Trichormus variabilis. The heterocyst and akinete formation was significantly decreased after exposure to extract containing 2 or 20 nM of microcystins within 10 days of exposure. To our knowledge, this is the first information about effects of metabolites produced by planktonic cyanobacteria on cell differentiation in filamentous cyanobacteria. Since the effects were induced by environmentally relevant concentrations of microcystins, our observations may indicate not only that microcystins or other bioactive peptides could affect conservation, overwintering, and nitrogen-fixation in filamentous cyanobacteria under environmental conditions but also contribute to the understanding of possible signaling function of cyanobacterial metabolites.