Expression of WWOX protein in non-small cell lung cancer and its significance

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Objective To study the protein expression level of the tumor suppressor gene WWOX in non-small cell lung cancer (NSCLC) and its clinical significance.Methods The protein expression of WWOX gene in paraffin-embedded specimens of 40 cases of NSCLC and 21 cases of adjacent normal lung tissue was detected by two stage method of immunohistochemistry.Results ①The positive rate of WWOX protein expression was higher in adjacent normal lung tissue than that in NSCLC tissue (85.71 ％vs 37.50％,P＜0.05),and there was no obvious difference in the protein expression of WWOX between squamous carcinoma and adenocarcinoma (34.62 ％ vs 42.86 ％,P ＞0.05).②The protein positive rate of WWOX was lower in male patients than that in females (25.00％ vs 56.25％,P ＜0.05),and the protein expression of WWOX was lower in patients with smoking index≥400 than patients with smoking index＜400 (19.05％ vs 57.89％,P ＜ 0.05).There was no significant difference in mean age between the patients whose WWOX protein expression was positive and those whose WWOX protein expression was negative ( P ＞ 0.05).③ There was no significant difference in WWOX expression among the poorly,medium and well differentiated cancer tissue ( P ＞0.05).④The protein expression of WWOX in NSCLC had no significant difference between the patients with stage Ⅰ + Ⅱ and stage Ⅲ + Ⅳ ( P ＞0.05).Whether there was metastasis or not,the WWOX protein expression in NSCLC was consistent.Conclusions WWOX protein expression in NSCLC tissue is lower than that in adjacent normal lung tissue,and is related to gender and smoking index,but has no obvious relationship with age,pathological type,degree of differentiation,clinical stage and metastasis. Key words: Non-small cell lung cancer; WWOX; Immunohistochemistry