Abstract
Objective To prepare, purify and characterize the polyclonal antibody against SLC35F2,and detect the expression of SLC35F2 protein in non-small-cell lung cancer (NSCLC).Methods Four polypeptides named peptide 1,2,3 and 4 were synthesized based on the bioinformatics analysis of SLC35F2.The mixed polypeptides were injected into New Zealand rabbits.The polyclonal antibody was purified by immunoaffinity chromatography, and identified by Western blot and immunohistochemistry.The expression of SLC35F2 protein in 129 cases of NSCLC and corresponding adjacent normal lung tissues was detected by tissue microarray with immunohistochemistry.Results The titers of polyclonal antibody were 1:105 detected by ELISA assay.Western blot confirmed its high specific 41 KD band.The expression of SLC35F2 protein was strongly positive (++ to +++) in90.7% (117/129) of the NSCLC samples.On the contrast, only 17.1% (22/129) adjacent normal lung tissues were strongly positive (++).The expression of SLC35F2 protein was significantly higher in NSCLC than in the adjacent normal lung tissues (P <0.01).Correlation analysis revealed that pathological stage had a low correlation with the expression of SLC35F2 protein.Factor analyses showed that 93.3% expression of SLC35F2 protein in NSCLC tissues were correlated with tumor differentiation and stage.Conclusion The specific antibody against human SLC35F2 was obtained.SLC35F2 protein was highly expressed in most of the NSCLC. Key words: Non small celllung cancer; Homo sapiens solute carrier family 35 member F2; Polyclonal antibody
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