Abstract
Objective To verify weather kidney injury molecular-1 (KIM-1 could reflect the function of rat kidney undergoing warm ischemia injury (WII).Methods An ischemia reperfusion model was established.Based on the length of warm ischemia time,rats were divided into control group (no WII),15-min WII group,30-min WII group,45-min WII group and 60-min WII group.After reperfusion for 8 h,allograft samples were collected and cut into sections for hematoxylin and eosin (HE) staining,immunohistochemistry and Western blotting,respectively.Blood was collected for further tests.Results With the extension of the warm ischemia time,the kidney tubules became swollen and necrotic.Cr went from (32.1 ±5.7) μmol/L to (96.8 ±9.5) μmol/L,but cumulative absorbance value (IA) of KIM-1 went from 372 ± 62 to 40 928 ± 238.The KIM-1 expression was correlated with Cr (r =0.91,P ＜ 0.01).Conclusion As a biomarker,the expression of KIM-1 get stronger and stranger with the the extension of warm ischemia time.In combination with pathological examination,KIM-1 could reflect function of the kidney under WII. Key words: Kidney injury molecular-1 ; Warm Ischemia; Reperfusion injury
Published Version
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