Protective effects of hydrodynamics-based injection preconditioning on hepatic warm ischemia and reperfusion injury in mice

Abstract

Objective To investigate the effects of hydrodynamics-based injection preconditioning on hepatic warm ischemia and reperfusion (I/R) injury in mice.Methods Sixty male BALB/c mice were randomly divided into four groups:(1) sham group; (2) warm I/R group; (3) hydrodynamics-based injection (HD) + I/R group:Hydrodynamics-based injection (5-7 s) was performed at 72 h before isehemia with normal saline in a large volume of 2.5 mL; (4) routine injection (RT) + I/R group:Routine tail vein injection (＞30 s) was performed at 72 h before isehemia with normal saline in a large volume of 2.5 mL.The model of hepatic warm L/R was produced by clamping the hepatic portal for 45 min,followed by 6 h reperfusion.After reperfusion,blood and liver samples were collected.The levels of serum alanine aminotransferase (ALT),aspartate aminotransferase (AST) and hepatic malondialdehyde (MDA) were measured,and the histologic changes of the liver tissue were observed.Results The levels of serum ALT,AST and hepatic MDA in the HD + L/R group were (641.60 ±225.00) U/L,(1180.80 ±212.54) U/L,and (11.00 ± 1.06) mmol/L respectively,significantly lower than in RT + I/R group [(1716.20 ±283.50) U/L,(1994.60±303.80) U/L,(17.22 ±1.22) mmol/L] and L/R group [(1661.20±306.38) U/L,(2046.60±719.21) U/L,(17.20±2.09) mmol/L] (P＜0.05).Meanwhile,the histology revealed hepatocyte necrosis and infiltration of inflammatory cells were significantly alleviated in HD + I/R group.Conclusion Hydrodynamics-based injection preconditioning significantly attenuated hepatic warm I/R injury in mice. Key words: Tail vein injection; Hepatic; Warm ischemia; Reperfusion injury; Preconditioning